Un joli article sur les coccinelles suisses et la coccinelle asiatique Harmonia axyridis…PDF
par S. Fasolin, paru dans Landliebe Juni/Juli 2012
THOMAS A, TROTMAN J, WHEATLEY A, AEBI A, ZINDEL R and BROWN PMJ(2013) Predation of native coccinellids by the invasive alien Harmonia axyridis (Coleoptera: Coccinellidae): detection in Britain by PCR-based gut analysis. Insect Conservation and Diversity 6:20-27 PDF
The process of biological control carries a distinct risk that an alien biological control agent (BCA) will become established as an invasive alien species with an associated threat to the local ecosystem biodiversity. It is imperative that a wide-ranging environmental risk assessment (ERA) is performed before the release of any BCA. This should include considering various potential but difficult to observe ecological interactions between the BCA and members of the native community, including disruption of intraguild relationships. Detection of intraguild predation (IGP) events involving predatory arthropods in the field can be done by analyzing their gut contents. Polymerase chain reaction (PCR) is a sensitive and specific tool to identify target prey DNA within a predator’s gut. This paper reviews the efficiency of a DNA based approach for detecting IGP in the field, compared with detection by the use of monoclonal antibodies or gas chromatography. Prey specificity, detection times after prey consumption, capacity for quantification, multiple prey targeting and the time and costs involved in developing and using the different methods are considered.
Aebi A, Brown PMJ, de Clercq P, Hautier L, Howe AG, Ingels B, Ravn HP, Sloggett JJ, Zindel R and Thomas A (2011) Measuring arthropod intraguild predation in the field. BioControl 56:429–440 DOI 10.1007/s10526-011-9378-2